Investigating cultivation strategies for enhancing protein content in Auxenochlorella pyrenoidosa FACHB-5.

This study investigated the influence of yeast extract addition, carbon source, and photoperiod on the growth dynamics of Auxenochlorella pyrenoidosa FACHB-5. Employing response surface methodology, the culture strategy was optimized, resulting in the following optimal conditions: yeast extract addition at 0.75 g L-1, glucose concentration of 0.83 g L-1, and a photoperiod set at Light: Dark = 18 h: 6 h. Under these conditions, the biomass reached 1.76 g L-1 with a protein content of 750.00 g L-1, containing 40 % of essential amino acids, representing a 1.52-fold increase. Proteomic analysis revealed that the targeted cultivation strategy up-regulated genes involved in microalgal protein synthesis. The combined effect of yeast extract and glucose enhanced both the glutamine synthetase-glutamate synthetase mechanism and the free amino acid content.

A modified cultivation strategy to enhance biomass production and lipid accumulation of Tetradesmus obliquus FACHB-14 with copper stress and light quality induction.

In this study, a two-stage culture strategy was refined to concurrently enhance the growth and lipid accumulation of Tetradesmus obliquus. The results unveiled that, during the initial stage, the optimal conditions for biomass accumulation were achieved with 0.02 mg·L-1 Cu2+ concentration and red light. Under these conditions, biomass accumulation reached 0.628 g·L-1, marking a substantial 23.62 % increase compared to the control group. In the second stage, the optimal conditions for lipid accumulation were identified as 0.5 mg·L-1 Cu2+ concentration and red light, achieving 64.25 mg·g-1·d-1 and marking a 128.38 % increase over the control. Furthermore, the fatty acid analysis results revealed an 18.85 % increase in the saturated fatty acid content, indicating enhanced combustion performance of microalgae cultivated under the dual stress of red light and 0.5 mg·L-1 Cu2+. This study offers insights into the potential application of Tetradesmus obliquus in biofuel production.

Study on the removal and degradation mechanism of microcystin-LR by the UV/Fenton system.

Microcystin-LR (MC-LR) is highly hepatotoxic and potentially carcinogenic to humans. Therefore, removing MC-LR from water bodies is of paramount importance. This study aimed to investigate the removal efficacy of the UV/Fenton system on MC-LR from copper-green microcystin and to explore its degradation mechanism in simulated real algae-containing wastewater. The results showed that at an initial concentration of 5 µg·L-1, a combination of 300 µmol·L-1 H2O2, 125 µmol·L-1 FeSO4, and 5 min of UV irradiation under average radiation intensity of 48 µW·cm-2 resulted in a removal efficiency of 90.65 % for MC-LR. The reduction of extracellular soluble microbial metabolites of Microcystis aeruginosa confirmed the UV/Fenton method's degradation efficiency for MC-LR, while the observation of the functional group CH and OCO in the treatment group indicated effective binding sites in the coagulation process. However, the presence of humic substances in algal organic matter (AOM) and some proteins and polysaccharides in the algal cell suspension competed with MC-LR for HO·, resulting in a decreased removal effect (78.36 %) in simulated actual algae-containing wastewater. These quantitative results provide an experimental basis and theoretical foundation for controlling cyanobacterial water blooms and ensuring drinking water quality safety.

Low-frequency ultrasound and nitrogen limitation induced enhancement in biomass production and lipid accumulation of Tetradesmus obliquus FACHB-12.

The two-stage cultivation strategy was optimized in this study to simultaneously promote the growth and lipid accumulation of Tetradesmus obliquus. Results showed that the optimal dual-stress conditions were nitrogen concentration at 25 mg N·L-1 and low-frequency ultrasound at 200 Watt, 1 min, and 8 h interval. The biomass and lipid content of Tetradesmus obliquus were increased by 32.1% and 44.5%, respectively, comparing to the control, and the lipid productivity reached 86.97 mg-1·L-1·d-1 at the end of the cultivation period. The protein and photosynthetic pigment contents of microalgae decreased by 22.4% and 14.0% under dual stress comparing to the control environment. In addition, dual stress cultivation of microalgae presented higher level of antioxidant capacity to balance to oxidation level in microalgal cells. This study provides a new insight for microalgae growth and lipid accumulation with dual stress stimulation.

A comprehensive review on carbon source effect of microalgae lipid accumulation for biofuel production.

Energy is a major driving force for the economic development. Due to the scarcity of fossil fuels and negative impact on the environment, it is important to develop renewable and sustainable energy sources for humankind. Microalgae as the primary feedstock for biodiesel has shown great application potential. However, lipid yield from microalgae is limited by the upstream cost, which restrain the realization of large-scale biofuel production. The modification of lipid-rich microalgae cell has become the focus over the last few decades to improve the lipid content and productivity of microalgae. Carbon is a vital nutrient that regulates the growth and metabolism of microalgae. Different carbon sources are assimilated by microalgae cells via different pathways. Inorganic carbon sources are mainly used through the CO2-concentrating mechanisms (CCMs), while organic carbon sources are absorbed by microalgae mainly through the Pentose Phosphate (PPP) Pathway and the Embden-Meyerhof-Pranas (EMP) pathway. Therefore, the addition of carbon source has a significant impact on the production of microalgae biomass and lipid accumulation. In this paper, mechanisms of lipid synthesis and carbon uptake of microalgae were introduced, and the effects of different carbon conditions (types, concentrations, and addition methods) on lipid accumulation in microalgal biomass production and biodiesel production were comprehensively discussed. This review also highlights the recent advances in microalgae lipid cultivation with large-scale commercialization and the development prospects of biodiesel production. Current challenges and constructive suggestions are proposed on cost-benefit concerns in large-scale production of microalgae biodiesel.

Feasibility and comparative analysis of cadmium biosorption by living scenedesmus obliquus FACHB-12 biofilms.

Microalgal biofilm has been recognized as a cost-effective biorsorbent for heavy metal and a promising method for microalgae-water separation. In this study, living suspended Scenedesmus obliquus FACHB-12 (isolated from southern China) and its biofilm with different carriers were investigated to remove cadmium from aqueous solution. S. obliquus FACHB-12 biofilm with luffa sponge carrier showed highest cadmium removal efficiency at 92.7% compared to biofilm with K3 carrier (75.3%) and significantly higher than suspended S. obliquus FACHB-12 (61.8%) in 2 h experiment with initial Cd2+ concentration at 3.0 mg/L at pH = 6.0 with 0.8 g/L of biomass under room temperature. S. obliquus FACHB-12 biofilm with K3 and luffa sponge carrier also demonstrated higher tolerance towards increased Cd2+ concentration with highest biosorption efficiency at 85.1% and 90.35% respectively under 20 mg/L of Cd2+, while suspended S. obliquus FACHB-12 biosorption efficiency achieved 81.4% under 10 mg/L of Cd2+ and started to decline over increased cadmium concentration. The adsorption kinetics for all experimental groups followed the pseudo-second-order adsorption model, with biosorption equilibrium favored in Langmuir isotherm. The maximum biosorption capacity estimated by Langmuir isotherm reached 133.14 mg/g biomass in S. obliquus FACHB-12 biofilm with luffa sponge carrier, followed by 78.76 mg/g with K3 carrier, and 60.03 mg/g with suspended S. obliquus FACHB-12. Results suggest an efficient, inexpensive microalgal biofilm with biological carrier system could enhance high cadmium removal for advanced wastewater treatment and provide a cost-effective method for microalgae harvesting process.

Microelectrode Investigation on the Corrosion Initiation at Lead-Brass Galvanic Interfaces in Chlorinated Drinking Water.

In this study, the effects of pH, dissolved inorganic carbon (DIC), and flow on changes in surface chemistry (pH, dissolved oxygen, and free chlorine) of lead-brass joints at initial stages of corrosion were investigated using microelectrodes. Surface measurements showed that the water chemistry at the metal surfaces was highly heterogeneous. At pH 7 and during water stagnation, local pH difference between anodic (leaded-solder) and cathodic (brass) regions differed by as much as 7.5 pH units. High DIC water under the water flowing condition showed minimal pH changes on the surface, whereas in low DIC water, a pH range of 7.6-5.4 (ΔpH 2.2) was observed over the surface. Free chlorine consumption near the lead-brass surface was greater under stagnation, regardless of bulk pH. It was also found that flow can move the low pH plume that originated at the anode. Overall, this study provides direct evidence for highly localized galvanic corrosion in a chlorinated drinking water environment.

Emerging pollutants in water environment: Occurrence, monitoring, fate, and risk assessment.

The occurrence of emerging pollutants (EPs) is continuously reported worldwide. Nevertheless, only few of these compounds are toxicologically evaluated due to their vast numbers. Reliable analytical methods and toxicity assessment methods are the basis of either the management or the elimination of EPs. In this paper, literature published in 2018 on EPs were reviewed with special regard to their occurrence, detection methods, fate in the environment, and ecological toxicity assessment. Particular focus was placed on practical considerations, novel processes, and new solution strategies. PRACTITIONER POINTS: Literature published in 2018 on emerging pollutants were reviewed. This review article is with special regard to the occurrence, detection methods, fate and toxicity assessment of emerging pollutants. Particular focus was placed on practical considerations, novel processes and new solution strategies.

In Situ Monitoring of Pb2+ Leaching from the Galvanic Joint Surface in a Prepared Chlorinated Drinking Water.

A novel method using a micro-ion-selective electrode (micro-ISE) technique was developed for in situ lead monitoring at the water-metal interface of a brass-leaded solder galvanic joint in a prepared chlorinated drinking water environment. The developed lead micro-ISE (100 µm tip diameter) showed excellent performance toward soluble lead (Pb2+) with sensitivity of 22.2 ± 0.5 mV decade-1 and limit of detection (LOD) of 1.22 × 10-6 M (0.25 mg L-1). The response time was less than 10 s with a working pH range of 2.0-7.0. Using the lead micro-ISE, lead concentration microprofiles were measured from the bulk to the metal surface (within 50 µm) over time. Combined with two-dimensional (2D) pH mapping, this work clearly demonstrated that Pb2+ ions build-up across the lead anode surface was substantial, nonuniform, and dependent on local surface pH. A large pH gradient (ΔpH = 6.0) developed across the brass and leaded-tin solder joint coupon. Local pH decreases were observed above the leaded solder to a pH as low as 4.0, indicating it was anodic relative to the brass. The low pH above the leaded solder supported elevated lead levels where even small local pH differences of 0.6 units (ΔpH = 0.6) resulted in about four times higher surface lead concentrations (42.9 vs 11.6 mg L-1) and 5 times higher fluxes (18.5 × 10-6 vs 3.5 × 10-6 mg cm-2 s-1). Continuous surface lead leaching monitoring was also conducted for 16 h.

In-situ 2D maps of pH shifts across brass-lead galvanic joints using microelectrodes.

Galvanic corrosion in drinking water distribution systems, such as conditions following partial lead (Pb) service line replacement, has received recent attention. In order to better understand conditions at galvanic connections that lead to enhanced metal release and provide remedial strategies, the water-metal and anodic-cathodic interfaces at these locations must be better understood. In this paper, a pH microelectrode system was used to create in-situ 2D spatial images of the pH of water across two brass coupons connected by a leaded solder joint at 100 µm above the metal's surface under flowing and stagnation conditions. Water stagnation resulted in significant pH changes across the surfaces compared to flow condition. Under stagnation, the pH above the anode (leaded solder) was 1.5 pH units below the bulk water and as much as 2.5 units below the cathode (brass). These conditions can enhance lead release at the anode, which reflects different anodic-cathodic relationships of coupled metals primarily controlled by water flow. Most importantly, this work has demonstrated the ability to make real pH measurement at the surface of corroding metals using a novel microelectrode approach.